quantitative high-resolution mass spectrometry-based approach (massarray) platform Search Results


proteomics wildtype b vulgatus atcc 8482  (ATCC)
97
ATCC proteomics wildtype b vulgatus atcc 8482
(A) Growth curves of B. vulgatus in defined medium with and without various retinoids. The horizontal dashed line indicates the threshold used for calculating time-to-log phase measurements. (B,C) Bar plots indicating mean (± SEM) retinoid sensitivity, calculated as time-to-log phase for treated cultures versus time-to-log phase for vehicle alone (DMSO) control cultures for (B) B. vulgatus <t>ATCC</t> <t>8482</t> versus B. dorei DSM 17855 or (C) B. vulgatus strain ATCC 8482 versus B. vulgatus strain 257_H4 isolated from a healthy Malawian infant (16). For example, the sensitivity value of 20.8±2.1 for wildtype B. vulgatus incubated in medium containing 10 µM retinol in panel B was calculated by dividing the total incubation period (in this case 95 h) by the time required for vehicle alone-treated control cultures of the same strain to cross the OD600 threshold of 0.3. Mean values ± SEM are shown except under those conditions in panel C where the concentration of retinol tested completely inhibited growth. n=2 independent experiments, each in triplicate for panel B and one experiment performed in triplicate for panel C. *P<0.05, **, P<0.01, ***, P<0.001 (one-way ANOVA, Bonferroni multiple comparisons test).
Proteomics Wildtype B Vulgatus Atcc 8482, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cavity ring 124 down spectrometer  (Picarro Inc)
97
Picarro Inc cavity ring 124 down spectrometer
(A) Growth curves of B. vulgatus in defined medium with and without various retinoids. The horizontal dashed line indicates the threshold used for calculating time-to-log phase measurements. (B,C) Bar plots indicating mean (± SEM) retinoid sensitivity, calculated as time-to-log phase for treated cultures versus time-to-log phase for vehicle alone (DMSO) control cultures for (B) B. vulgatus <t>ATCC</t> <t>8482</t> versus B. dorei DSM 17855 or (C) B. vulgatus strain ATCC 8482 versus B. vulgatus strain 257_H4 isolated from a healthy Malawian infant (16). For example, the sensitivity value of 20.8±2.1 for wildtype B. vulgatus incubated in medium containing 10 µM retinol in panel B was calculated by dividing the total incubation period (in this case 95 h) by the time required for vehicle alone-treated control cultures of the same strain to cross the OD600 threshold of 0.3. Mean values ± SEM are shown except under those conditions in panel C where the concentration of retinol tested completely inhibited growth. n=2 independent experiments, each in triplicate for panel B and one experiment performed in triplicate for panel C. *P<0.05, **, P<0.01, ***, P<0.001 (one-way ANOVA, Bonferroni multiple comparisons test).
Cavity Ring 124 Down Spectrometer, supplied by Picarro Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quantitative high-resolution mass spectrometry-based approach (massarray) platform  (agena bioscience)
90
agena bioscience quantitative high-resolution mass spectrometry-based approach (massarray) platform
(A) Growth curves of B. vulgatus in defined medium with and without various retinoids. The horizontal dashed line indicates the threshold used for calculating time-to-log phase measurements. (B,C) Bar plots indicating mean (± SEM) retinoid sensitivity, calculated as time-to-log phase for treated cultures versus time-to-log phase for vehicle alone (DMSO) control cultures for (B) B. vulgatus <t>ATCC</t> <t>8482</t> versus B. dorei DSM 17855 or (C) B. vulgatus strain ATCC 8482 versus B. vulgatus strain 257_H4 isolated from a healthy Malawian infant (16). For example, the sensitivity value of 20.8±2.1 for wildtype B. vulgatus incubated in medium containing 10 µM retinol in panel B was calculated by dividing the total incubation period (in this case 95 h) by the time required for vehicle alone-treated control cultures of the same strain to cross the OD600 threshold of 0.3. Mean values ± SEM are shown except under those conditions in panel C where the concentration of retinol tested completely inhibited growth. n=2 independent experiments, each in triplicate for panel B and one experiment performed in triplicate for panel C. *P<0.05, **, P<0.01, ***, P<0.001 (one-way ANOVA, Bonferroni multiple comparisons test).
Quantitative High Resolution Mass Spectrometry Based Approach (Massarray) Platform, supplied by agena bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quantitative high-resolution mass spectrometry-based approach (massarray) platform  (Sequenom)
90
Sequenom quantitative high-resolution mass spectrometry-based approach (massarray) platform
(A) Growth curves of B. vulgatus in defined medium with and without various retinoids. The horizontal dashed line indicates the threshold used for calculating time-to-log phase measurements. (B,C) Bar plots indicating mean (± SEM) retinoid sensitivity, calculated as time-to-log phase for treated cultures versus time-to-log phase for vehicle alone (DMSO) control cultures for (B) B. vulgatus <t>ATCC</t> <t>8482</t> versus B. dorei DSM 17855 or (C) B. vulgatus strain ATCC 8482 versus B. vulgatus strain 257_H4 isolated from a healthy Malawian infant (16). For example, the sensitivity value of 20.8±2.1 for wildtype B. vulgatus incubated in medium containing 10 µM retinol in panel B was calculated by dividing the total incubation period (in this case 95 h) by the time required for vehicle alone-treated control cultures of the same strain to cross the OD600 threshold of 0.3. Mean values ± SEM are shown except under those conditions in panel C where the concentration of retinol tested completely inhibited growth. n=2 independent experiments, each in triplicate for panel B and one experiment performed in triplicate for panel C. *P<0.05, **, P<0.01, ***, P<0.001 (one-way ANOVA, Bonferroni multiple comparisons test).
Quantitative High Resolution Mass Spectrometry Based Approach (Massarray) Platform, supplied by Sequenom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quantitative high-resolution mass spectrometry-based approach (massarray) platform - by Bioz Stars, 2026-04
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mass spectrometry  (Sequenom)
90
Sequenom mass spectrometry
(A) Growth curves of B. vulgatus in defined medium with and without various retinoids. The horizontal dashed line indicates the threshold used for calculating time-to-log phase measurements. (B,C) Bar plots indicating mean (± SEM) retinoid sensitivity, calculated as time-to-log phase for treated cultures versus time-to-log phase for vehicle alone (DMSO) control cultures for (B) B. vulgatus <t>ATCC</t> <t>8482</t> versus B. dorei DSM 17855 or (C) B. vulgatus strain ATCC 8482 versus B. vulgatus strain 257_H4 isolated from a healthy Malawian infant (16). For example, the sensitivity value of 20.8±2.1 for wildtype B. vulgatus incubated in medium containing 10 µM retinol in panel B was calculated by dividing the total incubation period (in this case 95 h) by the time required for vehicle alone-treated control cultures of the same strain to cross the OD600 threshold of 0.3. Mean values ± SEM are shown except under those conditions in panel C where the concentration of retinol tested completely inhibited growth. n=2 independent experiments, each in triplicate for panel B and one experiment performed in triplicate for panel C. *P<0.05, **, P<0.01, ***, P<0.001 (one-way ANOVA, Bonferroni multiple comparisons test).
Mass Spectrometry, supplied by Sequenom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mass spectrometry - by Bioz Stars, 2026-04
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massarray system  (Sequenom)
90
Sequenom massarray system
(A) Growth curves of B. vulgatus in defined medium with and without various retinoids. The horizontal dashed line indicates the threshold used for calculating time-to-log phase measurements. (B,C) Bar plots indicating mean (± SEM) retinoid sensitivity, calculated as time-to-log phase for treated cultures versus time-to-log phase for vehicle alone (DMSO) control cultures for (B) B. vulgatus <t>ATCC</t> <t>8482</t> versus B. dorei DSM 17855 or (C) B. vulgatus strain ATCC 8482 versus B. vulgatus strain 257_H4 isolated from a healthy Malawian infant (16). For example, the sensitivity value of 20.8±2.1 for wildtype B. vulgatus incubated in medium containing 10 µM retinol in panel B was calculated by dividing the total incubation period (in this case 95 h) by the time required for vehicle alone-treated control cultures of the same strain to cross the OD600 threshold of 0.3. Mean values ± SEM are shown except under those conditions in panel C where the concentration of retinol tested completely inhibited growth. n=2 independent experiments, each in triplicate for panel B and one experiment performed in triplicate for panel C. *P<0.05, **, P<0.01, ***, P<0.001 (one-way ANOVA, Bonferroni multiple comparisons test).
Massarray System, supplied by Sequenom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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high-resolution mass spectrometry  (Shotgun Proteomics company)
90
Shotgun Proteomics company high-resolution mass spectrometry
(A) Growth curves of B. vulgatus in defined medium with and without various retinoids. The horizontal dashed line indicates the threshold used for calculating time-to-log phase measurements. (B,C) Bar plots indicating mean (± SEM) retinoid sensitivity, calculated as time-to-log phase for treated cultures versus time-to-log phase for vehicle alone (DMSO) control cultures for (B) B. vulgatus <t>ATCC</t> <t>8482</t> versus B. dorei DSM 17855 or (C) B. vulgatus strain ATCC 8482 versus B. vulgatus strain 257_H4 isolated from a healthy Malawian infant (16). For example, the sensitivity value of 20.8±2.1 for wildtype B. vulgatus incubated in medium containing 10 µM retinol in panel B was calculated by dividing the total incubation period (in this case 95 h) by the time required for vehicle alone-treated control cultures of the same strain to cross the OD600 threshold of 0.3. Mean values ± SEM are shown except under those conditions in panel C where the concentration of retinol tested completely inhibited growth. n=2 independent experiments, each in triplicate for panel B and one experiment performed in triplicate for panel C. *P<0.05, **, P<0.01, ***, P<0.001 (one-way ANOVA, Bonferroni multiple comparisons test).
High Resolution Mass Spectrometry, supplied by Shotgun Proteomics company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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6495 triple quadrupole mass spectrometer ifunnel  (Agilent technologies)
90
Agilent technologies 6495 triple quadrupole mass spectrometer ifunnel
(A) Growth curves of B. vulgatus in defined medium with and without various retinoids. The horizontal dashed line indicates the threshold used for calculating time-to-log phase measurements. (B,C) Bar plots indicating mean (± SEM) retinoid sensitivity, calculated as time-to-log phase for treated cultures versus time-to-log phase for vehicle alone (DMSO) control cultures for (B) B. vulgatus <t>ATCC</t> <t>8482</t> versus B. dorei DSM 17855 or (C) B. vulgatus strain ATCC 8482 versus B. vulgatus strain 257_H4 isolated from a healthy Malawian infant (16). For example, the sensitivity value of 20.8±2.1 for wildtype B. vulgatus incubated in medium containing 10 µM retinol in panel B was calculated by dividing the total incubation period (in this case 95 h) by the time required for vehicle alone-treated control cultures of the same strain to cross the OD600 threshold of 0.3. Mean values ± SEM are shown except under those conditions in panel C where the concentration of retinol tested completely inhibited growth. n=2 independent experiments, each in triplicate for panel B and one experiment performed in triplicate for panel C. *P<0.05, **, P<0.01, ***, P<0.001 (one-way ANOVA, Bonferroni multiple comparisons test).
6495 Triple Quadrupole Mass Spectrometer Ifunnel, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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massarray epityper  (agena bioscience)
90
agena bioscience massarray epityper
(A) Growth curves of B. vulgatus in defined medium with and without various retinoids. The horizontal dashed line indicates the threshold used for calculating time-to-log phase measurements. (B,C) Bar plots indicating mean (± SEM) retinoid sensitivity, calculated as time-to-log phase for treated cultures versus time-to-log phase for vehicle alone (DMSO) control cultures for (B) B. vulgatus <t>ATCC</t> <t>8482</t> versus B. dorei DSM 17855 or (C) B. vulgatus strain ATCC 8482 versus B. vulgatus strain 257_H4 isolated from a healthy Malawian infant (16). For example, the sensitivity value of 20.8±2.1 for wildtype B. vulgatus incubated in medium containing 10 µM retinol in panel B was calculated by dividing the total incubation period (in this case 95 h) by the time required for vehicle alone-treated control cultures of the same strain to cross the OD600 threshold of 0.3. Mean values ± SEM are shown except under those conditions in panel C where the concentration of retinol tested completely inhibited growth. n=2 independent experiments, each in triplicate for panel B and one experiment performed in triplicate for panel C. *P<0.05, **, P<0.01, ***, P<0.001 (one-way ANOVA, Bonferroni multiple comparisons test).
Massarray Epityper, supplied by agena bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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liquid high-resolution bruker avance-iii nmr spectrometer  (Bruker Corporation)
90
Bruker Corporation liquid high-resolution bruker avance-iii nmr spectrometer
(A) Growth curves of B. vulgatus in defined medium with and without various retinoids. The horizontal dashed line indicates the threshold used for calculating time-to-log phase measurements. (B,C) Bar plots indicating mean (± SEM) retinoid sensitivity, calculated as time-to-log phase for treated cultures versus time-to-log phase for vehicle alone (DMSO) control cultures for (B) B. vulgatus <t>ATCC</t> <t>8482</t> versus B. dorei DSM 17855 or (C) B. vulgatus strain ATCC 8482 versus B. vulgatus strain 257_H4 isolated from a healthy Malawian infant (16). For example, the sensitivity value of 20.8±2.1 for wildtype B. vulgatus incubated in medium containing 10 µM retinol in panel B was calculated by dividing the total incubation period (in this case 95 h) by the time required for vehicle alone-treated control cultures of the same strain to cross the OD600 threshold of 0.3. Mean values ± SEM are shown except under those conditions in panel C where the concentration of retinol tested completely inhibited growth. n=2 independent experiments, each in triplicate for panel B and one experiment performed in triplicate for panel C. *P<0.05, **, P<0.01, ***, P<0.001 (one-way ANOVA, Bonferroni multiple comparisons test).
Liquid High Resolution Bruker Avance Iii Nmr Spectrometer, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rtx-dioxin2 capillary column  (Restek Corporation)
90
Restek Corporation rtx-dioxin2 capillary column
(A) Growth curves of B. vulgatus in defined medium with and without various retinoids. The horizontal dashed line indicates the threshold used for calculating time-to-log phase measurements. (B,C) Bar plots indicating mean (± SEM) retinoid sensitivity, calculated as time-to-log phase for treated cultures versus time-to-log phase for vehicle alone (DMSO) control cultures for (B) B. vulgatus <t>ATCC</t> <t>8482</t> versus B. dorei DSM 17855 or (C) B. vulgatus strain ATCC 8482 versus B. vulgatus strain 257_H4 isolated from a healthy Malawian infant (16). For example, the sensitivity value of 20.8±2.1 for wildtype B. vulgatus incubated in medium containing 10 µM retinol in panel B was calculated by dividing the total incubation period (in this case 95 h) by the time required for vehicle alone-treated control cultures of the same strain to cross the OD600 threshold of 0.3. Mean values ± SEM are shown except under those conditions in panel C where the concentration of retinol tested completely inhibited growth. n=2 independent experiments, each in triplicate for panel B and one experiment performed in triplicate for panel C. *P<0.05, **, P<0.01, ***, P<0.001 (one-way ANOVA, Bonferroni multiple comparisons test).
Rtx Dioxin2 Capillary Column, supplied by Restek Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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orbitrap id-x mass spectrometer  (Thermo Fisher)
90
Thermo Fisher orbitrap id-x mass spectrometer
(A) Growth curves of B. vulgatus in defined medium with and without various retinoids. The horizontal dashed line indicates the threshold used for calculating time-to-log phase measurements. (B,C) Bar plots indicating mean (± SEM) retinoid sensitivity, calculated as time-to-log phase for treated cultures versus time-to-log phase for vehicle alone (DMSO) control cultures for (B) B. vulgatus <t>ATCC</t> <t>8482</t> versus B. dorei DSM 17855 or (C) B. vulgatus strain ATCC 8482 versus B. vulgatus strain 257_H4 isolated from a healthy Malawian infant (16). For example, the sensitivity value of 20.8±2.1 for wildtype B. vulgatus incubated in medium containing 10 µM retinol in panel B was calculated by dividing the total incubation period (in this case 95 h) by the time required for vehicle alone-treated control cultures of the same strain to cross the OD600 threshold of 0.3. Mean values ± SEM are shown except under those conditions in panel C where the concentration of retinol tested completely inhibited growth. n=2 independent experiments, each in triplicate for panel B and one experiment performed in triplicate for panel C. *P<0.05, **, P<0.01, ***, P<0.001 (one-way ANOVA, Bonferroni multiple comparisons test).
Orbitrap Id X Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Growth curves of B. vulgatus in defined medium with and without various retinoids. The horizontal dashed line indicates the threshold used for calculating time-to-log phase measurements. (B,C) Bar plots indicating mean (± SEM) retinoid sensitivity, calculated as time-to-log phase for treated cultures versus time-to-log phase for vehicle alone (DMSO) control cultures for (B) B. vulgatus ATCC 8482 versus B. dorei DSM 17855 or (C) B. vulgatus strain ATCC 8482 versus B. vulgatus strain 257_H4 isolated from a healthy Malawian infant (16). For example, the sensitivity value of 20.8±2.1 for wildtype B. vulgatus incubated in medium containing 10 µM retinol in panel B was calculated by dividing the total incubation period (in this case 95 h) by the time required for vehicle alone-treated control cultures of the same strain to cross the OD600 threshold of 0.3. Mean values ± SEM are shown except under those conditions in panel C where the concentration of retinol tested completely inhibited growth. n=2 independent experiments, each in triplicate for panel B and one experiment performed in triplicate for panel C. *P<0.05, **, P<0.01, ***, P<0.001 (one-way ANOVA, Bonferroni multiple comparisons test).

Journal: Science translational medicine

Article Title: The effects of micronutrient deficiencies on bacterial species from the human gut microbiota

doi: 10.1126/scitranslmed.aal4069

Figure Lengend Snippet: (A) Growth curves of B. vulgatus in defined medium with and without various retinoids. The horizontal dashed line indicates the threshold used for calculating time-to-log phase measurements. (B,C) Bar plots indicating mean (± SEM) retinoid sensitivity, calculated as time-to-log phase for treated cultures versus time-to-log phase for vehicle alone (DMSO) control cultures for (B) B. vulgatus ATCC 8482 versus B. dorei DSM 17855 or (C) B. vulgatus strain ATCC 8482 versus B. vulgatus strain 257_H4 isolated from a healthy Malawian infant (16). For example, the sensitivity value of 20.8±2.1 for wildtype B. vulgatus incubated in medium containing 10 µM retinol in panel B was calculated by dividing the total incubation period (in this case 95 h) by the time required for vehicle alone-treated control cultures of the same strain to cross the OD600 threshold of 0.3. Mean values ± SEM are shown except under those conditions in panel C where the concentration of retinol tested completely inhibited growth. n=2 independent experiments, each in triplicate for panel B and one experiment performed in triplicate for panel C. *P<0.05, **, P<0.01, ***, P<0.001 (one-way ANOVA, Bonferroni multiple comparisons test).

Article Snippet: High resolution quantitative mass spectrometry-based proteomics Wildtype B. vulgatus ATCC 8482 was cultured ( n =2 per treatment) in BDM in the presence of 1 µM retinol or vehicle alone (0.02% DMSO), pelleted, and frozen for storage at −80°C.

Techniques: Isolation, Incubation, Concentration Assay